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osteoblast like cell line saos 2  (ATCC)


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    Structured Review

    ATCC osteoblast like cell line saos 2
    Monophasic SrCPC and PLLA–PGA scaffolds and biphasic PLLA–PGA/SrCPC scaffolds seeded with <t>SAOS-2.</t> Printed scaffolds used for cell culture experiments; scale bars = 2 mm (a). Overview fluorescence microscopic images of scaffolds seeded with 5 × 10 5 cells/scaffold after 1 and 14 days of cultivation (b); adherent cells were stained with DAPI (cell nuclei; blue) and phalloidin (actin cytoskeletons; green), scale bars = 500 μm, C = cement (SrCPC), P = polymer (PLLA–PGA). Cell attachment and density on SrCPC and PLLA–PGA strands in monophasic and biphasic scaffolds seeded with 5 × 10 5 cells/scaffold (c) or 5 × 10 4 cells/scaffold (d). Detailed fluorescence microscopic images of scaffolds after 1 and 14 days of cultivation. Cells were stained with DAPI (cell nuclei; blue) and phalloidin (actin cytoskeletons; green). Scale bars = 100 μm.
    Osteoblast Like Cell Line Saos 2, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 3541 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/osteoblast+like+cell+line+saos+2/Saos-2/pmc13177265-83-1-5
    Average 98 stars, based on 3541 article reviews
    osteoblast like cell line saos 2 - by Bioz Stars, 2026-09
    98/100 stars

    Images

    1) Product Images from "Biphasic Bone Implants through Hybrid Extrusion Printing of Thermoplastic Poly(lactic- co -glycolic) acid and Strontium-Modified Calcium Phosphate Bone Cement"

    Article Title: Biphasic Bone Implants through Hybrid Extrusion Printing of Thermoplastic Poly(lactic- co -glycolic) acid and Strontium-Modified Calcium Phosphate Bone Cement

    Journal: ACS Omega

    doi: 10.1021/acsomega.5c12496

    Monophasic SrCPC and PLLA–PGA scaffolds and biphasic PLLA–PGA/SrCPC scaffolds seeded with SAOS-2. Printed scaffolds used for cell culture experiments; scale bars = 2 mm (a). Overview fluorescence microscopic images of scaffolds seeded with 5 × 10 5 cells/scaffold after 1 and 14 days of cultivation (b); adherent cells were stained with DAPI (cell nuclei; blue) and phalloidin (actin cytoskeletons; green), scale bars = 500 μm, C = cement (SrCPC), P = polymer (PLLA–PGA). Cell attachment and density on SrCPC and PLLA–PGA strands in monophasic and biphasic scaffolds seeded with 5 × 10 5 cells/scaffold (c) or 5 × 10 4 cells/scaffold (d). Detailed fluorescence microscopic images of scaffolds after 1 and 14 days of cultivation. Cells were stained with DAPI (cell nuclei; blue) and phalloidin (actin cytoskeletons; green). Scale bars = 100 μm.
    Figure Legend Snippet: Monophasic SrCPC and PLLA–PGA scaffolds and biphasic PLLA–PGA/SrCPC scaffolds seeded with SAOS-2. Printed scaffolds used for cell culture experiments; scale bars = 2 mm (a). Overview fluorescence microscopic images of scaffolds seeded with 5 × 10 5 cells/scaffold after 1 and 14 days of cultivation (b); adherent cells were stained with DAPI (cell nuclei; blue) and phalloidin (actin cytoskeletons; green), scale bars = 500 μm, C = cement (SrCPC), P = polymer (PLLA–PGA). Cell attachment and density on SrCPC and PLLA–PGA strands in monophasic and biphasic scaffolds seeded with 5 × 10 5 cells/scaffold (c) or 5 × 10 4 cells/scaffold (d). Detailed fluorescence microscopic images of scaffolds after 1 and 14 days of cultivation. Cells were stained with DAPI (cell nuclei; blue) and phalloidin (actin cytoskeletons; green). Scale bars = 100 μm.

    Techniques Used: Cell Culture, Fluorescence, Staining, Polymer, Cell Attachment Assay

    Cell growth and ALP activity of SAOS-2 cells cultured on monophasic SrCPC and PLLA–PGA scaffolds in comparison with biphasic PLLA–PGA/SrCPC scaffolds. Cell number was correlated with the cytosolic LDH activity measured after cell lysis (a), and the ALP activity as an indicator of osteogenic differentiation is shown as absolute activity per scaffold (b) and as specific activity in relation to the cell number (c) ( n = 3, mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).
    Figure Legend Snippet: Cell growth and ALP activity of SAOS-2 cells cultured on monophasic SrCPC and PLLA–PGA scaffolds in comparison with biphasic PLLA–PGA/SrCPC scaffolds. Cell number was correlated with the cytosolic LDH activity measured after cell lysis (a), and the ALP activity as an indicator of osteogenic differentiation is shown as absolute activity per scaffold (b) and as specific activity in relation to the cell number (c) ( n = 3, mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Techniques Used: Activity Assay, Cell Culture, Comparison, Lysis

    Related Articles

    Cell Culture:

    Article Title: A comparative analysis of 3D printed scaffolds consisting of poly(lactic- co -glycolic) acid and different bioactive mineral fillers: aspects of degradation and cytocompatibility.
    Article Snippet: Their excellent mechanical properties, degradability and suitability for processing by 3D printing technologies make the thermoplastic polylactic acid and its derivatives favourable candidates for biomaterialbased bone regeneration therapies.. In this study, we investigated whether bioactive mineral fillers, which are known to promote bone healing based on their dissolution products, can be integrated into a poly(Llactic-co-glycolic) acid (PLLA-PGA) matrix and how key characteristics of degradation and cytocompatibility are influenced.. The polymer powder was mixed with particles of CaCO3, SrCO3, strontium-modified hydroxyapatite (SrHAp) or tricalcium phosphates (α-TCP, β-TCP) in a mass ratio of 90 : 10; the resulting composite materials have been successfully processed into scaffolds by the additive manufacturing method Arburg Plastic Freeforming (APF).

    Polymer:

    Article Title: Poly(dl-lactide) Polymer Blended with Mineral Phases for Extrusion 3D Printing—Studies on Degradation and Biocompatibility
    Article Snippet: Inherent viscosity was measured using an Ubbelohde viscosimeter (PVS, Lauda Scientific; Königshofen, Germany) (K = 0.005 mm 2 /s 2 ); measurement was repeated two times. .. The osteoblast-like cell line SaOS-2 (ATCC 243; DSMZ, Braunschweig, Germany) was used to study the cytocompatibility of the polymer blends in direct contact culture as described previously [ ]. .. In brief, the cells were expanded in cell culture medium consisting of Minimum Essential Medium alpha modification (αMEM; Gibco, ThermoFisher Scientific, Waltham, MA, USA), 15% fetal calf serum (FCS; Corning, NY, USA), and 100 U mL −1 penicillin and 100 μg mL −1 streptomycin (PS).

    Adhesive:

    Article Title: Biological, mechanical and adhesive properties of universal adhesives containing zinc and copper nanoparticles.
    Article Snippet: Objectives: To evaluate the effect of addition of zinc oxide and copper nanoparticles (ZnO/CuNp) into universal adhesives, on antimicrobial activity (AMA), cytotoxicity (CTX), water sorption (WS) and solubility (SO), microhardness (MH) and in vitro degree of conversion (DC), as well as resin-dentin microtensile bond strength (μTBS), nanoleakage (NL) and in situ DC.. Methods: ZnO/CuNp (0% [control]; 5/0.1 and 5/0.2 wt%) were added in Prime&Bond Active (PBA) and Ambar Universal (AMB).. The AMA was evaluated against Streptococcus mutans.

    Activity Assay:

    Article Title: Biological, mechanical and adhesive properties of universal adhesives containing zinc and copper nanoparticles.
    Article Snippet: Objectives: To evaluate the effect of addition of zinc oxide and copper nanoparticles (ZnO/CuNp) into universal adhesives, on antimicrobial activity (AMA), cytotoxicity (CTX), water sorption (WS) and solubility (SO), microhardness (MH) and in vitro degree of conversion (DC), as well as resin-dentin microtensile bond strength (μTBS), nanoleakage (NL) and in situ DC.. Methods: ZnO/CuNp (0% [control]; 5/0.1 and 5/0.2 wt%) were added in Prime&Bond Active (PBA) and Ambar Universal (AMB).. The AMA was evaluated against Streptococcus mutans.



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    Image Search Results


    Monophasic SrCPC and PLLA–PGA scaffolds and biphasic PLLA–PGA/SrCPC scaffolds seeded with SAOS-2. Printed scaffolds used for cell culture experiments; scale bars = 2 mm (a). Overview fluorescence microscopic images of scaffolds seeded with 5 × 10 5 cells/scaffold after 1 and 14 days of cultivation (b); adherent cells were stained with DAPI (cell nuclei; blue) and phalloidin (actin cytoskeletons; green), scale bars = 500 μm, C = cement (SrCPC), P = polymer (PLLA–PGA). Cell attachment and density on SrCPC and PLLA–PGA strands in monophasic and biphasic scaffolds seeded with 5 × 10 5 cells/scaffold (c) or 5 × 10 4 cells/scaffold (d). Detailed fluorescence microscopic images of scaffolds after 1 and 14 days of cultivation. Cells were stained with DAPI (cell nuclei; blue) and phalloidin (actin cytoskeletons; green). Scale bars = 100 μm.

    Journal: ACS Omega

    Article Title: Biphasic Bone Implants through Hybrid Extrusion Printing of Thermoplastic Poly(lactic- co -glycolic) acid and Strontium-Modified Calcium Phosphate Bone Cement

    doi: 10.1021/acsomega.5c12496

    Figure Lengend Snippet: Monophasic SrCPC and PLLA–PGA scaffolds and biphasic PLLA–PGA/SrCPC scaffolds seeded with SAOS-2. Printed scaffolds used for cell culture experiments; scale bars = 2 mm (a). Overview fluorescence microscopic images of scaffolds seeded with 5 × 10 5 cells/scaffold after 1 and 14 days of cultivation (b); adherent cells were stained with DAPI (cell nuclei; blue) and phalloidin (actin cytoskeletons; green), scale bars = 500 μm, C = cement (SrCPC), P = polymer (PLLA–PGA). Cell attachment and density on SrCPC and PLLA–PGA strands in monophasic and biphasic scaffolds seeded with 5 × 10 5 cells/scaffold (c) or 5 × 10 4 cells/scaffold (d). Detailed fluorescence microscopic images of scaffolds after 1 and 14 days of cultivation. Cells were stained with DAPI (cell nuclei; blue) and phalloidin (actin cytoskeletons; green). Scale bars = 100 μm.

    Article Snippet: The osteoblast-like cell line SaOS-2 (ATCC 243; DSMZ, Braunschweig, Germany) was used to study the cell response to biphasic scaffolds in a direct contact culture.

    Techniques: Cell Culture, Fluorescence, Staining, Polymer, Cell Attachment Assay

    Cell growth and ALP activity of SAOS-2 cells cultured on monophasic SrCPC and PLLA–PGA scaffolds in comparison with biphasic PLLA–PGA/SrCPC scaffolds. Cell number was correlated with the cytosolic LDH activity measured after cell lysis (a), and the ALP activity as an indicator of osteogenic differentiation is shown as absolute activity per scaffold (b) and as specific activity in relation to the cell number (c) ( n = 3, mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Journal: ACS Omega

    Article Title: Biphasic Bone Implants through Hybrid Extrusion Printing of Thermoplastic Poly(lactic- co -glycolic) acid and Strontium-Modified Calcium Phosphate Bone Cement

    doi: 10.1021/acsomega.5c12496

    Figure Lengend Snippet: Cell growth and ALP activity of SAOS-2 cells cultured on monophasic SrCPC and PLLA–PGA scaffolds in comparison with biphasic PLLA–PGA/SrCPC scaffolds. Cell number was correlated with the cytosolic LDH activity measured after cell lysis (a), and the ALP activity as an indicator of osteogenic differentiation is shown as absolute activity per scaffold (b) and as specific activity in relation to the cell number (c) ( n = 3, mean ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).

    Article Snippet: The osteoblast-like cell line SaOS-2 (ATCC 243; DSMZ, Braunschweig, Germany) was used to study the cell response to biphasic scaffolds in a direct contact culture.

    Techniques: Activity Assay, Cell Culture, Comparison, Lysis